Figure 3.

LANP and E4F synergistically repress the E4 promoter. The effects of E4F and LANP on (A) pGL-E4 and (B) pGL-E4 mut promoter activity in CV-1 cells. The left side of each panel shows the activity expressed from pGL-E4 alone (black bars) or with increasing amounts (25, 50, 100, 150 ng) of E4F or LANP expression plasmids (colour shaded). The right side of each panel shows the effect of coexpression of E4F and LANP on pGL-E4 activity. The amount of E4F (E) and/or LANP (L) expression plasmid in each transfection is indicated by colour. The asterisk indicates synergistic repression. LANP, leucine-rich acidic nuclear protein.