Table 2.

Preparative isolation and purification of AOX from the mutant strains (on the example of CA2).

Stage	Result	Specific AOX activity, μmol·min-1·mg-1 of protein (U·mg-1)	Total AOX yield, U (%)*
Cell growth	17.7 g of cells	0.35 (per 1 mg of cells)	6130 (100%)
Cell disintegration	Crude extract, mg 2300	2.0	4600 (75%)
Fractionation by ammonium sulphate (30 – 70%)	Protein precipitate (70% of ammonium sulphate saturation), mg 750	4.0	3000 (49%)
Chromatography on DEAE – Toyopearl 650 M	Protein sample, mg 75	16.0	1200 (20%)

* 1 Unit is equal 1 μmol of converted substrate per 1 min at standard conditions of assay (20°C).