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The Tret1 gene is expressed mainly in the fat body of larvae of P. vanderplanki. In situ hybridization was carried out by using either antisense (A, C, E, and G) or sense (B, D, F, and H) riboprobes for Tret1 mRNA to hybridize to cross-sections of a dehydrating larvae in the thorax (A, B, E, and F) and posterior abdomen (C, D, G, and H). (Magnifications: A–D, ×100; E–H, ×400.) Rectangles in A–D indicate trimming areas for E–H. FB, fat body; Mg, midgut; Mu, muscle; SG, salivary gland.
