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. 2007 Jul 2;104(28):11585–11590. doi: 10.1073/pnas.0702538104

Fig. 4.

Fig. 4.

TRET1 is a facilitated transporter. (A) The pH dependency of TRET1. Xenopus oocytes expressing TRET1 were incubated in 105 mM trehalose at various pH conditions for 3 h. Each value is the mean ± SEM (n = 3). (B) The Na+ dependency of TRET1. Xenopus oocytes expressing TRET1 were incubated in 105 mM trehalose in either normal buffer (control) or Na+-free buffer (−Na+) for 3 h. Each value is the mean ± SEM (n = 3). (C) The effects of ionophores and an uncoupler on TRET1 activity. Xenopus oocytes expressing TRET1 were preincubated in either control buffer (0.1% acetone) or buffer containing 10 μM nigericin (Na+, K+ ionophore), 10 μM valinomycin (K+ ionophore), or 10 μM carbonylcyanide m-chlorophenylhydrazone (CCCP) (H+ ionophore, uncoupler) for 15 min, and then incubated in 105 mM trehalose with the corresponding ionophores or an uncoupler for 3 h. Each value is the mean ± SEM (n = 3).