Fig. 7.

TRET1 allows mammalian cells to increase trehalose uptake. Either the TRET1 expression vector (pPvTRET1-IRES2-AcGFP1) or vector only (pIRES2-AcGFP1) were transfected into CHO-K1, HuH-7, or NIH/3T3-3-4 cells. (A) Transfection efficiency was estimated from the ratio of AcGFP1-expressing cells analyzed by a flow cytometer. Each value is the mean ± SEM (n = 3). (B) The transfected cells were incubated in medium containing 100 mM trehalose for 3 h to determine trehalose uptake. Each value is the mean ± SEM (n = 3).