Fig. 3.

MCP-1 protein levels in fibroblast media as analysed by ELISA. Analysis was performed in conditioned media from fibroblasts under basal conditions (B), exposed to hypoxia (H), or reoxygenated 1 h after hypoxia (R). The mean ± s.d. levels of MCP-1 protein secreted by normoxic, hypoxic and reoxygenated cells in six different cells lines are represented (a). The mean values with (□) or without (▪) pretreatment with N-acetyl-L-cysteine (NAC) are represented (b). ELISA determinations were performed in three different samples from each cell line. Mean values were compared by Student's t-test for paired samples.