Fig. 4.

Migration of MonoMac1 monocytic cells in response to fibroblast-conditioned media. Migration index was calculated as the x-fold increase in migration observed over the negative control. Conditioned media were collected from fibroblasts under basal conditions (□) or exposed to hypoxia (▪) (6 h). The mean migration indices from six different fibroblast lines in normoxia and hypoxia are represented. The effect of preincubating media with anti-MCP-1 MoAb or isotype-matched mouse IgG1 is also shown. Error bars indicate the s.d. of the mean from six different fibroblast lines. Mean values were compared by Student's t-test. Statistically significant differences are indicated in Results.