Table 1.
Comparison of potency of different inducers of up-regulation of neutrophil adherence molecules
| Stimulus and concentrations | CD11b MFI | CD11c MFI | CD62L ratio |
|---|---|---|---|
| Reference strain NCTC 11638 | 179 ± 63 | 53 ± 2 | 0·13 |
| 100 μg/ml | (60) | (60) | (60) |
| Clinical strain 28i6 | 125 ± 59 | 43 ± 10 | – |
| 10 μg/ml | (60) | (60) | |
| Clinical strain 28i6 | 139 ± 62 | 55 ± 9 | 0·21 |
| 100μg/ml | (60) | (45) | (60) |
| PMA 10 ng/ml | 316 ± 16 | 43 ± 4 | 0·09 |
| (15) | (15) | (5) | |
| fMLP 10−8m | 228 ± 54 | 30 ± 7 | 0·17 |
| (45) | (30) | (30) |
The results are expressed as the mean fluorescence intensity (MFI) ± s.d. after substracting the values for unstimulated control cells for CD11b and CD11c, or as a ratio of the signals for stimulated and unstimulated control cells for CD62L. The time taken to reach maximum (or minimum) values, in minutes, is given in parentheses. The results are derived as the mean of three experiments with neutrophils from H. pylori-seronegative donors.