Fig. 2.

T cell clonality analysis of TCR Vα2 and Vα12 families by reverse transcription-polymerase chain reaction-single strand conformation polymorphism (RT-PCR-SSCP). Bulk cDNA was synthesized from peripheral blood mononuclear cells and TCR α genes carrying Vα2 and Vα12 were amplified by familial PCR. The amplified genes were electrophoresed on a non-denaturing gel to be separated according to their single strand conformations ((a) Vα2; (b) Vα12). The left lanes represent amplified genes from the cDNA of the systemic lupus erythematosus patient and the right lanes are those of a healthy donor.