Table 1.
Sequences of the TCR α and β genes identified by polymerase chain reaction (PCR) amplification of single cell-derived cDNAs
| α | β | ||||||
|---|---|---|---|---|---|---|---|
| Vα12 | nDn | Jα | Vβ2 | nDn | Jβ | ||
| YFCA | LSEAT | SGSA (J22) | 10/18 | FYIC | RAGQD | SYEQY (J2S7) | A22* |
| YFCA | LSEPT | NNAG (J39) | 3/18 | FYICSA | RAGGT | YNEOF(J2S1) | P39* |
| YFCA | LR | SGSA (J22) | 1/18 | ||||
| YFCA | LKAWAA | SGGG (J45) | 1/18 | ||||
| YFCA | L | RDDK (J30) | 1/18 | ||||
| YFCA | LSDY | NNND (J44) | 1/18 | ||||
| YFCA | LSEALRS | GSEK (J57) | 1/18 | ||||
TCR Vα12+ T cells were sorted at a ratio of one cell/well into a microtitre plate. TCR α genes were amplified from the single cell-derived cDNA in each well by PCR and their CDR3 regions were sequenced. TCR β genes of expanded TCR α clonotypes were analysed similarly.
*
The expanded T cell clonotypes were named as described.