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. 2001 Feb;123(2):210–218. doi: 10.1046/j.1365-2249.2001.01439.x

Fig. 1.

Fig. 1

Fractionation of TNF-α stimulating fractions. Separation of culture filtrate antigens using Prep cell as described. Pooled fractions were run on a 10% acrylamide gel, transferred to nitrocellulose paper and stained with aurodye. Each fraction was also monitored for its capacity to directly stimulate TNF-α in purified adherent cell population. All fractions were tested at a concentration of 0·1 μg/ml.