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Functional assessment of adherent cell assay for TNF-α release from two skin test-negative donors (TE and DH). Purified adherent cells (1 × 106 cells in 500 μl) were stimulated with either lipopolysaccharide (LPS; 0·1 μg), Fx10 (0·1 μg) or Fx24 (0·1 μg) in the presence or absence of Polymyxin B (PMB; 10 μg).
