Table 3a.
a. Effect of protein ‘A’ absorbtion of tuberculosis (TB) plasma on antigen-stimulated (Fx10) monocyte section of cytokines
| TNF-α | IL-6 | IL-10 | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Plasma ID | Pre | Post | % change | Pre | Post | % change | Pre | Post | % change |
| TB05 | 738 | 94 | −87·2 | 881 | 536 | −39·1 | 148 | 242 | +63·5 |
| TB09 | 364 | 66 | −81·8 | 674 | 820 | +26·7 | 122 | 146 | +19·6 |
| TB55 | 1026 | 290 | −71·7 | 1104 | 785 | −28·8 | 122 | 234 | +91·8 |
| TB90 | 656 | 180 | −72·5 | 1232 | 776 | −37 | 194 | 215 | +10·8 |
| TB149 | 1291 | 464 | −64 | 1539 | 1032 | −32·9 | 191 | 220 | +15·1 |
| TB153 | 1156 | 701 | −39·3 | 3107 | 1885 | −64·8 | 417 | 440 | +5·5 |
| TB157 | 1057 | 453 | − 7·1 | 2036 | 1225 | −39·8 | 98 | 172 | +75·5 |
| TB167 | 1362 | 556 | −59·1 | 2336 | 1127 | −51·7 | 116 | 140 | +20·6 |
| Mean (95% CI) | 605 (450–761) | 587 (190–983) | −50 (−81·276–18·974) | ||||||
| *P versus prior to protein A absorption | P < 0·0001 | P < 0·009 | P < 0·006 | ||||||
Expression of all TNF-α and IL-6 was significantly decreased following absorption of plasma using protein A. A trend towards increased expression of IL-10 is evident.
*
Significance determined by paired t-test using Fisher's two-tailed analysis.