Table 3b.
b. Effect of protein ‘A’ absorbtion of TB plasma on antigen-stimulated (Fx24) monocyte section of cytokines
| TNF-α | IL-6 | IL-10 | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Plasma ID | Pre | Post | % change | Pre | Post | % change | Pre | Post | % change |
| TB05 | 88 | 0 | −100 | 276 | 9·8 | −96·4 | 58 | 132 | +127 |
| TB09 | 350 | 0 | −100 | 326 | 143 | −56 | 140 | 175 | +25 |
| TB55 | 211 | 9·1 | −95·6 | 276 | 84·5 | −69 | 87 | 148 | +70 |
| TB90 | 83 | 0 | −100 | 511 | 184 | −63·9 | 148 | 135 | −8·7 |
| TB149 | 742 | 16·1 | −17·8 | 873 | 359 | −58·8 | 154 | 194 | +25·9 |
| TB153 | 287 | 305 | 6·27 | 2573 | 193 | −92·4 | 63 | 550 | +773 |
| TB157 | 585 | 4·5 | −99·2 | 1394 | 359 | −74·2 | 180 | 159 | −11·6 |
| TB167 | 632 | 32·5 | −94·8 | 671 | 316 | −52·9 | 25 | 57·5 | +130 |
| Mean (95% CI) | 326 (92·1–560) | 656 (30·3–1282) | − 60·7 (− 224·8–50·94) | ||||||
| *P versus control | P = 0·013 | P = 0·04 | P = 0·17 | ||||||
Expression of all TNF-α and IL-6 was significantly decreased following absorption of plasma using protein A. A trend towards increased expression of IL-10 is evident.
*
Significance determined by paired t-test using Fisher's two-tailed analysis.