Table 3.

Kinetics of influx of ⁴⁵Ca²⁺ into N-formyl-l-methionyl-l-leucyl-l-phenylalanine (FMLP)-activated control and chronic granulomatous disease (CGD) neutrophils

	Influx of Ca2+ into FMLP-activated neutrophils (pmoles cell-associated Ca2+/107 neutrophils)
Time after addition	Contro lneutrophils (n = 6)	CGD neutrophils (n = 6)
10 s	0	1 ± 1
20 s	0	18 ± 10
30 s	4 ± 4	34 ± 16
60 s	16 ± 8	54 ± 9*
2 min	56 ± 9	107 ± 18*
3 min	109 ± 13	166 ± 13*
5 min	156 ± 16	167 ± 15

The results are expressed as the mean values for six different control subjects and the four different CGD patients with repeat evaluations performed on DT and RS.

^*P < 0·05 – P < 0·001 for comparison between the uptake of ⁴⁵Ca²⁺ by control and CGD cells at the corresponding times.

The amount of influx of Ca²⁺ into unstimulated control and CGD neutrophils at 5 min was 17 ± 6 and 27 ± 4 pmol/10⁷ cells, respectively.