Fig. 5.

Exogenously added IL-4 does not inhibit the downregulation of CD30 on human TCC. Th0 (29 : 19, 25 : 4) (□) and Th2 clones (24 : 9, 12 : 3, 19 : 3) (•) were activated for 16 h with anti-CD3 antibodies in serum-free medium (1 × 10⁶ cells in 1 ml medium) supplemented with IL-2 (20 U/ml) and with 1 mm NAC, 1 mm NAC + IL-4 (1000 U/ml), 5 mm NAC, 5 mm NAC + IL-4 (1000 U/ml), 20 mm NAC and 20 mm NAC + IL-4 (1000 U/ml). The CD30 expression was analysed with flow cytometry using the anti-CD30 MoAb Ber-H2 (Dakopatts). The addition of hrIL-4 (1000 U/ml) did not change the expression of CD30 significantly. Results are shown as decrease in CD30 MFI (% of control). The central line shows the mean value. The MFI values with no addition of NAC (control) were 157 and 83 in the Th0 clones, and 276, 58 and 124 in the Th2 clones.