Fig. 8.
Effect of DN-MEK on complement-induced protection in COS-7 cells. COS-7 cells after transfection (▪) or nontransfected cells (□) were treated with a sublytic dose of antibody and HI-NHS or NHS. Next, the cells were subjected to lytic antibody and NHS serially diluted 1:8–1:32 in PBS. Percent protection in induced cells (treated with NHS) was calculated relative to noninduced cells (treated with HI-NHS). Results are expressed as the mean percent protection + SD (n = 3). The experiment is representative of 3 independent experiments.