Fig. 4.

Competition assay between the recombinant protein by truncated KMP11 proteins and the 12642 and 12638 synthetic peptides. The serum samples (a) BF (b) RP and (c) PRM that reacted against 12638 and 12642 peptides were preincubated at a 1 : 1600 dilution with increasing amounts of either the KMP11 protein (○), the amino-terminal truncated KMP11 protein (□), the carboxyl-terminal truncated KMP11 protein (▴) or an equimolecular amount of the synthetic peptide 12642 which corresponds to the carboxyl-terminal region of the T. cruzi KMP11 protein (▪) and the synthetic peptide 12638 which corresponds to the central region of the protein (•). The data represent the mean of values obtained in three independent experiments (SD ≤ 5%). The inhibition molar ratios (moles of inhibitor/moles of coating antigen) were calculated considering the amount of KMP11 antigen (40 pmol) used to coat the ELISA wells.