Fig. 4.

In vitro SAA degradation by PBMC pretreated with LPS or cytokines. A: PBMC were pretreated with LPS (10 µg/ml), IL-1 β (20 u/ml) or IFN-γ (200u/ml) in 96 well plates for 24 h. Then SAA was added and cultured for another 12 h. Culture supernatants were subjected to anti-SAA immunoblot analysis. B: PBMC were pretreated with IL-1 β (20 u/ml), IFN-γ (200u/ml), TNF-α (200u/ml) or IL-6 (200u/ml) in 96 well plates for 24 h. Then SAA was added and cultured for another 12 h. Culture supernatants were subjected to anti-SAA immunoblot analysis. A representative example of three independent experiments showing similar results.