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. 2001 Apr;124(1):157–162. doi: 10.1046/j.1365-2249.2001.01503.x

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© 2001 Blackwell Science Ltd

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Fig. 2 — Dapsone inhibits the IL-8 release from NHEK, induced by IgG from a BP patient, in a dose-dependent manner. NHEK were incubated with a combination of 4 mg/ml IgG, affinity-purified from the BP patient's serum, and various concentrations of dapsone (BP IgG + dapsone) or nicotinamide (BP IgG + nicotinamide) for 12 h. IL-8 levels after stimulation with BP IgG alone (BP IgG), IgG from a healthy volunteer (Normal IgG), and culture medium alone (Medium) are shown on the left. IL-8 levels in the culture supernatant were analysed in quadruplicate by ELISA. Bars show mean ±SD (pg/ml). Asterisks indicate a statistical significant difference of the IL-8 release compared to BP IgG-treated NHEK (P < 0·01). This pattern is representative of the patterns seen in three separate experiments with keratinocytes from different donors. □ BP IgG; Normal IgG; ▪ Medium.

Inline graphic — Dapsone inhibits the IL-8 release from NHEK, induced by IgG from a BP patient, in a dose-dependent manner. NHEK were incubated with a combination of 4 mg/ml IgG, affinity-purified from the BP patient's serum, and various concentrations of dapsone (BP IgG + dapsone) or nicotinamide (BP IgG + nicotinamide) for 12 h. IL-8 levels after stimulation with BP IgG alone (BP IgG), IgG from a healthy volunteer (Normal IgG), and culture medium alone (Medium) are shown on the left. IL-8 levels in the culture supernatant were analysed in quadruplicate by ELISA. Bars show mean ±SD (pg/ml). Asterisks indicate a statistical significant difference of the IL-8 release compared to BP IgG-treated NHEK (P < 0·01). This pattern is representative of the patterns seen in three separate experiments with keratinocytes from different donors. □ BP IgG; Normal IgG; ▪ Medium.