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. 2001 Apr;124(1):157–162. doi: 10.1046/j.1365-2249.2001.01503.x

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© 2001 Blackwell Science Ltd

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Fig. 4 — Dapsone does not inhibit the BP180-mediated increase of IL-8 mRNA levels in NHEK as analysed by RT PCR. NHEK were treated with 4 mg/ml R594 (raised against recombinant human BP180 NC16A), preimmune rabbit IgG (Normal rabbit IgG), medium without any IgG (Medium), and with the combination of R594 IgG together with various dapsone concentrations (left side). In addition, NHEK were stimulated with 4 mg/ml IgG affinity-purified from a BP patient (BP IgG) or from a healthy volunteer (Normal human IgG), with medium alone (Medium), and with the combination of patient's IgG together with various dapsone concentrations (right side). Threshold cycles (Ct) for amplification of IL-8 mRNA are presented, bars show means of triplicate determinations ±SD. RT-PCR analysis was performed semiquantitatively. A difference in Ct of 3·5 cycles corresponds to a 10-fold difference in mRNA concentration. Asterisks indicate a statistical significant difference of the IL-8 release compared to R594 or BP IgG treated NHEK (P < 0·01). □ Anti-BP180 IgG; Normal IgG; ▪ Medium.

Inline graphic — Dapsone does not inhibit the BP180-mediated increase of IL-8 mRNA levels in NHEK as analysed by RT PCR. NHEK were treated with 4 mg/ml R594 (raised against recombinant human BP180 NC16A), preimmune rabbit IgG (Normal rabbit IgG), medium without any IgG (Medium), and with the combination of R594 IgG together with various dapsone concentrations (left side). In addition, NHEK were stimulated with 4 mg/ml IgG affinity-purified from a BP patient (BP IgG) or from a healthy volunteer (Normal human IgG), with medium alone (Medium), and with the combination of patient's IgG together with various dapsone concentrations (right side). Threshold cycles (Ct) for amplification of IL-8 mRNA are presented, bars show means of triplicate determinations ±SD. RT-PCR analysis was performed semiquantitatively. A difference in Ct of 3·5 cycles corresponds to a 10-fold difference in mRNA concentration. Asterisks indicate a statistical significant difference of the IL-8 release compared to R594 or BP IgG treated NHEK (P < 0·01). □ Anti-BP180 IgG; Normal IgG; ▪ Medium.