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. 2001 Apr;124(1):110–117. doi: 10.1046/j.1365-2249.2001.01494.x

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© 2001 Blackwell Science Ltd

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Fig. 1 — Depletion of γδ T cells from PBMNC abrogated the increase in both the induction of sIgA on B cells and the production of IgA in IgAN. Whole PBMNC or PBMNC depleted of γδ T cells obtained from IgAN patients (n = 10) and controls (n = 10) were cultured with 5 µg/ml PWM for 7 days. The proportions of B cells expressing each class of Ig were analysed using FACScan. The levels of each class of Ig in culture supernatant were measured by ELISA. □ 0 days; 7 days; ▪ 7 days without γδ T cells.The proportions of sIgA+ B cells after cultivation were higher in PBMNC from IgAN patients than in PBMNC from the controls (*P < 0·01). Depletion of γδ T cells from PBMNC obtained from IgAN patients significantly decreased the induction of sIgA on B cells (*P < 0·01). Similarly, the levels of IgA in culture supernatants of PBMNC from IgAN patients were higher than those in culture supernatants of PBMNC from the controls (*P < 0·01), and they were decreased by the removal of γδ T cells (*P < 0·01).

Inline graphic — Depletion of γδ T cells from PBMNC abrogated the increase in both the induction of sIgA on B cells and the production of IgA in IgAN. Whole PBMNC or PBMNC depleted of γδ T cells obtained from IgAN patients (n = 10) and controls (n = 10) were cultured with 5 µg/ml PWM for 7 days. The proportions of B cells expressing each class of Ig were analysed using FACScan. The levels of each class of Ig in culture supernatant were measured by ELISA. □ 0 days; 7 days; ▪ 7 days without γδ T cells.The proportions of sIgA+ B cells after cultivation were higher in PBMNC from IgAN patients than in PBMNC from the controls (*P < 0·01). Depletion of γδ T cells from PBMNC obtained from IgAN patients significantly decreased the induction of sIgA on B cells (*P < 0·01). Similarly, the levels of IgA in culture supernatants of PBMNC from IgAN patients were higher than those in culture supernatants of PBMNC from the controls (*P < 0·01), and they were decreased by the removal of γδ T cells (*P < 0·01).