Table 2.
Junctional sequences of Vγ9 clones from IgAN patients
| Patient | V-region | D-region | J-region | Number of clones | Clones obtained repeatedly from different donors |
|---|---|---|---|---|---|
| 1 | TYYCALWEV | QEDER | ..ELGKKIKVFGPGTKLIIT | 4/12 | *1 |
| 2 | TYYC..... | CLPQG | .QELGKKIKVFGPGTKLIIT | 3/12 | |
| TYYC..... | RILPQG | ..ELGKKIKVFGPGTKLIIT | 2/12 | ||
| 3 | TYYCALWE. | GG | ..ELGKKIKVFGPGTKLIIT | 4/12 | *2 |
| TYYCALWE. | PL | .QELGKKIKVFGPGTKLIIT | 2/12 | ||
| 4 | TYYCAL... | VT | NYYKKLFGSGTTLVVT | 3/12 | *3 |
| TYYCALWE. | GG | ..ELGKKIKVFGPGTKLIIT | 3/12 | *2 | |
| 5 | TYYCALWEV | QEDER | ..ELGKKIKVFGPGTKLIIT | 3/12 | *1 |
| TYYCALWE. | DA | .QELGKKIKVFGPGTKLIIT | 2/12 | ||
| 6 | TYYCAL... | VT | NYYKKLFGSGTTLVVT | 3/12 | *3 |
| TYYCALWE. | DRR | ....KLFGSGTTLVVT | 2/12 | ||
| 7 | TYYCAL... | PSPD | ...KKLFGSGTTLVVT | 12/12 | *4 |
| 8 | TYYCALWE. | GG | ..ELGKKIKVFGPGTKLIIT | 4/12 | *2 |
| TYYCALWEV | QEDER | ..ELGKKIKVFGPGTKLIIT | 3/12 | *1 | |
| 9 | TYYCAL... | PSPD | ...KKLFGSGTTLVVT | 11/12 | *4 |
| 10 | TYYCALWEV | L | ..ELGKKIKVFGPGTKLIIT | 2/12 | |
| TYYCALW. | RT | ..ELGKKIKVFGPGTKLIIT | 2/12 | ||
| 11 | TYYCAL... | PSPD | ...KKLFGSGTTLVVT | 12/12 | *4 |
| 12 | TYYC..... | LQVPQG | ..ELGKKIKVFGPGTKLIIT | 2/12 |
The cDNAs of Vγ9 gene segments were amplified by RT-PCR and cloned and then their sequences were determined. At least 12 clones from each IgAN patient (n = 12) were analysed. The deduced amino acid sequences of the most frequent clones in each IgAN patient are shown. Numbers on the right
*
1 to *4 indicate the clones that were obtained repeatedly from different donors.