Fig. 5.
LPG inhibits HIV-1 replication. Sup-T1 cells (1 × 106) were infected with HIV-1NL4–3 (100 ng of p24 gag) in the absence (a) or presence (b) of LPG (10 µm). After an incubation period of 7 days, cells were photographed at a magnification of 100× with an inverted microscope. Arrows indicate the presence of syncytia. 1G5 cells (1 × 105) were infected with HIV-1NL4-3 (10 ng of p24 gag) in the presence (▪) or absence (□) of Leishmania LPG (5 µm) for 48 h (c). In some experiments, HIVNL4-3 (10 ng of p24) was first incubated in the presence or the absence of Leishmania LPG (5 µm) before inoculation of 1G5 cells (1 × 105) for 48 h (d). Finally, cells were lysed and assayed for luciferase activity as described in Materials and Methods. Results shown are the mean ±SD of each treatment from triplicates. This is representative of two independent experiments.