Fig. 6.
LPG acts at a postbinding step on the HIV-1 replicative cycle. (a) LPG-treated or untreated Jurkat cells (1 × 105) were incubated in the presence of HIV-1NL4-3 (10 ng of p24 gag) at 4°C for 30 min. After extensive washing, cells were lysed and p24 quantification was performed. Controls consisted of either uninfected or Jurkat cells treated with sCD4 before inoculation with viruses. (b) LPG-treated or untreated Jurkat cells (1 × 105) were incubated in the presence of HIV-1NL4-3 (100 ng of p24 gag) at 37°C for 2·5 h. and then treated with pronase (0·1 mg/ml) for 5 min. Controls consisted of samples not treated with pronase or samples which were incubated with the virus at 4°C prior to the addition of pronase. Standard p24 quantification was subsequently performed after cell lysis. Results shown represent the mean ±SD for each sample carried out in triplicate. These data are representative of three independent experiments.