ELISA reactivity of immune and preimmune sera from mice immunized with βA4[35–43]-DT. (a) Responses of mice to βA4[35–43] peptide used as the immunogen (mouse 1 (□), mouse 2 (Δ), mouse 3 (∇)). (b,c,d) Reactivity of these antisera to βA4[1–40], βA4[1–42] and βA4[1–43], respectively. Preimmune sera are delineated by the following symbols: mouse 1 (▪); mouse 2 (▴); and mouse 3 (▾).