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The influence of growth conditions on MBL binding to F. necrogenes. Organims were grown under various conditions and the binding of MBL was then assayed. (a) Overnight growth on blood agar (control). (b) Growth for 24 h in fastidious anaerobe broth (FAB). (c) Growth for 2 h in FAB. (d) Growth for 2 h in FAB, then heat killed at 56°C for 30 min. The percentage of organisms positive for MBL binding is identified in each panel.
