Fig. 2.

Schematic representation of the genetic elements used to generate the three MCP (CD46) transgenic mouse strains. The upper part of the figure shows the part of chromosome 1, band 1q32, that contains the regulators of complement activation (RCA) gene cluster. The gene sizes and intergenic distances of the members of the RCA cluster are drawn to scale and gene orientations are designated 5′ to 3′. Below are shown the two YAC clones used to generate the three transgenic mouse strains. Both YACs contain a ∼400 kb genomic insert that includes the entire MCP (CD46) gene. While the alignment of YAC RCA1 with chromosome 1 is precise, the alignment of YAC AM2 : 6C10 is only approximate as indicated by the dotted parentheses. The insertion sites of the YACs in the mouse genomes are not known. For the designation and accession numbers of the two YAC clones see Materials and methods.