Fig. 7.
Detection of human MCP (CD46) on E of the three transgenic mouse strains. E were purified from whole mouse and human blood samples by centrifugation through Histopaque gradients. After hypotonic lysis, membranes were solubilized and an equivalent of 1 × 105 cells was separated by SDS-PAGE under nonreducing conditions. The samples were characterized by Western blotting using a polyclonal antihuman-MCP (CD46) Ab. Mixtures of recombinant isoforms of human MCP (CD46) (i.e. BC1/BC2 and C1/C2) expressed in CHO cells were used as controls. The relative sharp bands seen in E preparations of the three transgenic strains tested imply a distinct glycosylation pattern.