Table 3.
Effects of chemotherapy on absolute cell numbers of splenic B cell subpopulations
| Treatment | |||||
|---|---|---|---|---|---|
| Day | Control | CP | MTX | CyPh | |
| Absolute cell numbers | 686 ± 188·9 | ||||
| per spleen | 2 | 438·3 ± 34·3* | 496·4 ± 46·3 | 300·7 ± 64·4* | |
| 7 | 580·8 ± 143·1 | 611·6 ± 25·3 | 383·8 ± 90·5* | ||
| 15 | 517·7 ± 54·3 | 998·1 ± 55·9* | 818·7 ± 209·7 | ||
| 24 | 721·9 ± 22·5 | 379·4 ± 41·0* | 395·4 ± 94·4* | ||
| NF-B | 21·7 ± 10·3 | ||||
| 2 | 8·0 ± 3·9* | 8·4 ± 0·8* | 5·8 ± 1·7* | ||
| 7 | 3·0 ± 0·5* | 7·0 ± 1·9* | 0·9 ± 0·5* | ||
| 15 | 18·2 ± 2·1 | 15·7 ± 5·8 | 30·5 ± 11·9 | ||
| 24 | 23·0 ± 2·6 | 7·8 ± 2·5* | 11·7 ± 3·8 | ||
| EFR-B | 32·8 ± 11·0 | ||||
| 2 | 15·0 ± 6·2* | 18·9 ± 0·2* | 13·2 ± 3·3* | ||
| 7 | 8·9 ± 1·6* | 14·8 ± 0·9* | 4·7 ± 2·8* | ||
| 15 | 28·2 ± 2·7 | 35·1 ± 8·3 | 56·5 ± 14·7* | ||
| 24 | 37·7 ± 1·2 | 13·8 ± 3·2* | 23·1 ± 7·3 | ||
| RF-B | 76·1 ± 18·2 | ||||
| 2 | 69·5 ± 30·7 | 66·3 ± 8·7 | 19·4 ± 6·5* | ||
| 7 | 106·1 ± 31·4 | 93·7 ± 8·9 | 30·6 ± 5·0* | ||
| 15 | 87·5 ± 10·6 | 129·1 ± 18·2* | 34·4 ± 6·8* | ||
| 24 | 92·7 ± 5·0 | 50·7 ± 14·4 | 36·4 ± 8·2* | ||
| MZ-B | 26·3 ± 8·9 | ||||
| 2 | 25·7 ± 10·7 | 16·9 ± 4·4 | 5·1 ± 1·4* | ||
| 7 | 24·8 ± 4·4 | 23·1 ± 6·0 | 7·8 ± 2·3* | ||
| 15 | 20·2 ± 1·6 | 21·9 ± 4·4 | 9·7 ± 3·7* | ||
| 24 | 17·9 ± 0·5 | 9·9 ± 1·3* | 8·8 ± 3·0* | ||
Effects on B cell populations were analysed by three-colour flow cytometry using IgM, CD90 and HIS57. Absolute cell numbers (×106 ± standard deviation) were determined by multiplying the frequency of the B cell subpopulation by the absolute number of cells per spleen.
*
Indicates a significant difference with control values (P < 0·05)