Table 4.
Antibody binding characteristics of the three groups of monoclonal antibodies and their effects on in vitro coagulation
| Anti-β2GPI | Anticardiolipin (direct ELISA) | Anti- prothrombin | Lupus anticoagulant | Thrombin inhibition | |
|---|---|---|---|---|---|
| Group 1 | 11/13 (85%) | 3/18 (16%) | 0/13 (0%) | 7/15 (46%) | 18/18 (100%) |
| Group 2 | 1/17 (6%) | 10/17 (59%) | 0/7 (0%) | 1/14 (7%) | 1/17 (6%) |
| Group 3 | 4/5 (80%) | 2/5 (40%) | 1/4 (25%) | 1/4 (25%) | 4/5 (80%) |
Group 1: 18 IgG monoclonal antibodies from BALB/c mice immunized with human β2GPI. Group 2: 17 IgG autoimmune monoclonal antibodies from NZW × BXSB F1 mice. Group 3 : five IgM autoimmune monoclonal antibodies from three patients with antiphospholipid syndrome. Note that not all tests were able to be performed with all MoAbs as the quantities available were limited.