Fig. 3.

Effects of phenytoin treatment on cytokine production. KLH-specific IL-4 (a) and IFN-γ (b) production by splenoctyes from phenytoin-treated mice. Splenocytes (5 × 10⁶/ml) from control (□) or phenytoin-treated (▪) mice immunized with KLH were stimulated with 50 or 100 µg/ml KLH for 18 h and culture supernatants were harvested. Cytokines were detected by ELISA. Results are expressed as mean ± SE of cytokines (pg or ng/ml) produced by 5 × 10⁶ splenocytes (n = 5). *significantly different. c, Serum level of IFN-γ in phenytoin-treated mice. Control (□) or phenytoin-treated (▪) mice were injected i.p. with LPS or PBS and their sera were harvested 18 h later. IFN-γ levels in their sera were detected by ELISA. Results are expressed as mean ± SE (n = 5). * significantly different.