Fig. 2.

Involvement of plastic-adherent cells in early impairment of pig islet cell (PIC) function by spleen cells from NOD mice. (a) Insulin release from 100 000 PIC cultured for 48 h alone (•), with 1·5 × 10⁶ whole mouse spleen cells (○), or with 1·5 × 10⁶ mouse spleen cells depleted of plastic-adherent cells (□). Each point indicates the mean value ±s.e.m. of 10 experiments. P < 0·01 between PIC cultured with whole spleen cells or spleen cells depleted of plastic-adherent cells. (b) Insulin release from PIC cultured for 48 h alone (•) or with 300 000 mouse plastic-adherent cells (○). Each point indicates the mean value ±s.e.m. of 10 experiments. P < 0·01 between PIC cultured alone or with plastic-adherent spleen cells. (c) Insulin release from PIC cultured for 48 h alone (•), with supernatant fluids from previous co-cultures of PIC and mouse spleen cells (○), or with plastic-adherent spleen cells (□). Each point indicates the mean value ±s.e.m. of three experiments. P < 0·01 between PIC cultured alone or with supernatant fluids. (d) Effects of 0·01 µg/ml (▪), 1 µg/ml (◊), or 100 µg/ml (□) of gadolinium chloride on insulin release from PIC co-cultured with mouse spleen cells for 48 h. Comparisons were performed with co-cultures of PIC and spleen cells without gadolinium chloride (○) or with PIC alone (•). Each point indicates the mean value ±s.e.m. of three experiments. P < 0·01 between PIC cultured with mouse spleen cells in the absence and presence of Gad. The grey area corresponds to the glucose stimulation period.