Amino acid sequence, SDS-PAGE and immunoblotting of N- and C-terminal subunits of EgEF-1β/δ. The two subunits were subjected to 12% SDS-PAGE and stained with Coomassie Brilliant Blue (lanes 1 and 2) or transferred to nitrocellulose and revealed with the monoclonal antibody specific to the 6× histidine (lanes 3, 6), human serum IgE (lanes 4, 7) and human serum IgG (lanes 5, 8). Molecular weights (MW) are indicated on the left.