Fig. 3.

Western blot analysis of periplasmic preparations of HB2151 containing scFv inserts. One colony from each set of electroporation which had an intact scFv insert as determined from Fig. 4 was grown and induced with IPTG for scFv secretion. The periplasmic preparation was electrophoresed through a 15% SDS-polyacrylamide gel and transferred onto PVDF membrane. The c-myc tag at the 3′ end of scFvs was detected using the monoclonal antibody 9E10. All the colonies produced scFv as evidenced by bands at the correct position (approximately 26 kD), indicated by the arrow.