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Concentration of the fusion proteins III-20-Fc, IV-67-Fc and Fc control.
| Clone | Concentration |
|---|---|
| III-20-Fc | 600 ng/ml |
| IV-67-Fc | 3500 ng/ml |
| Fc | 1200 ng/ml |
The concentrations were determined by sandwich ELISA. Mouse anti-human IgG1at 10 µg/ml was used as the capture antibody coated in the solid phase. Purified human IgG1 was used to prepare the standard curve from which the concentrations of the recombinant proteins were determined.
