Fig. 5.

p65 and p65/p50 stimulate the expression of a reporter vector containing six copies of the CD40L NF-κB binding site. A hexamer of the CD40L NF-κB binding site was placed upstream of the TKm promoter, in the luciferase pGL3 reporter plasmid. p65 and p50 expression plasmids were co-transfected with this reporter plasmid into Jurkat cells; 20 h after the transfection, Jurkat cells were stimulated with PMA (50 ng/ml) and ionomycin (1 µm). The experiment was performed in triplicate and the intra-assay variation was less than 20%.