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. 2001 Aug;125(2):283–290. doi: 10.1046/j.1365-2249.2001.01598.x

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© 2001 Blackwell Science Ltd

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Fig. 5 — Effect of E-64-d on the abnormal down-regulation of PKC activity in Con A-stimulated PBMC from CHS patients and normal controls. The PBMC (1×10⁶/ml) from four CHS (P_1,2,3,4) and two normal controls (C_1,2) were incubated with E-64-d (1 μg/ml) or medium alone for 1 h. Then the cells were stimulated with or without Con A (20 μg/ml) for 20 min. The membrane-bound (a) and the cytosolic (b) PKC activity was assayed as described in Patients and methods. ▪ Medium; □ Con A; E-64-d + Con A.

Inline graphic — Effect of E-64-d on the abnormal down-regulation of PKC activity in Con A-stimulated PBMC from CHS patients and normal controls. The PBMC (1×10⁶/ml) from four CHS (P_1,2,3,4) and two normal controls (C_1,2) were incubated with E-64-d (1 μg/ml) or medium alone for 1 h. Then the cells were stimulated with or without Con A (20 μg/ml) for 20 min. The membrane-bound (a) and the cytosolic (b) PKC activity was assayed as described in Patients and methods. ▪ Medium; □ Con A; E-64-d + Con A.