Table 2.

Effects of E-64-d and other reagents on lysosomal enzyme activity in CHS and normal cell lines

			Enzyme activity
Cell line	Reagent	Concentration	Elastase (U/106 cells)	Cathepsin G (U/106 cells)	β-Galactosidase (%)	Collagenase- like peptidase (%)
Normal	Medium		40·0±2·32	0·11±0.01	100	100
	Chelerythrin	1 μm	25·2±0·80*	0·06±0.01*	92·9	93·6
	C2-ceramide	3 μg/ml	16·0±1·92*	0·03±0.004*	92·8	89·6
	E-64-d	1 μg/ml	38·4±1·28	0·09±0.008	99·8	99·5
CHS	Medium		11·2±0·96*	0·04±0.02*	100	100
	E-64-d	1 μg/ml	33·2±2·52†	0·09±0.02†	97·5	98·4

After cells were cultured overnight in the presence of indicated reagents, the cell extracts were assayed for lysosomal enzyme activity as described in Patients and methods. One unit of elastase and cathepsin G activity is expressed as nmol products/h. Data are expressed as means ± s.e. of at least three experiments. The activity of β-galactosidase and collagenase-like peptidase is expressed as means of the percentage of the enzyme activity of each cell line cultured with medium alone. No significant difference in the activity of these enzymes was observed between normal and CHS cell lines.

^*P < 0·01, significant when compared with normal cell lines with medium alone.

^†P < 0·01, significant when compared with CHS cell lines with medium alone.