Fig. 2.

Effect of anti-FcγRI and/or anti-FcγRII on IFN-γ-induced MHC class II expression. PBMC (1 × 10⁶ cells/ml) were incubated with either medium or anti-FcγRI (5 µg/ml) and/or anti-FcγRII (5 µg/ml) for 1 h at 4°C. Then, the cells were washed and incubated with medium or (Fab′)₂ anti‐mouse IgG (10 µg/ml) for 1 h at 37°C. After this period, the cells were washed and incubated with medium or IFN-γ (240 U/ml) for 24 h and stained with anti-HLA-DR and anti-CD14 antibodies. Data are expressed as percentage MFI of control cells and correspond to the CD14⁺ population of PBMC. The inhibition of class II antigen by IC (100 µg/ml) in the same experimental conditions was 63%. The figure shows one representative experiment of n = 5.