Fig. 3.

Effect of different amounts of IC on basal and IFN-γ-induced MHC class II expression. PBMC (1 × 10⁶ cells/ml) were incubated with medium (control) or different ratios of IC as indicated in the figure (1 : 1 corresponds to the concentration 100 µg/ml). After 1 h at 37°C, the cells were incubated with (a) IFN-γ (240 U/ml) or (b) medium for 24 h. After this period, PBMC were stained with anti-HLA-DR and anti-CD14 antibodies and evaluated by flow cytometry. Data are expressed as percentage MFI ± s.e.m. of control cells and correspond to the CD14⁺ population of PBMC, n = 3.