Fig. 5.

MHC class I presentation of rNP in the absence of TAP and the effect of inhibitors of the conventional MHC class I pathway and endosomal antigen processing. BM28·7 and BM36·1 cells were incubated with 5 or 50 µm rNP derived from influenza virus A/HK/2/68 or infected with influenza virus (m.o.i. = 0·1) (a), incubated with various concentrations of the HLA-B27-restricted peptide SRYWAIRTR (b), or incubated with 50 µm rNP derived from influenza virus A/HK/2/68 in the presence of lactacystin or brefeldin A (c). Cells were used as target cells in CTL assays with the NP/B27 CTL as effector cells. Experiments were performed with 10 replicates per target per experiment. Mean percentages of specific lysis at an E:T ratio of 10:1 are shown. BM28·7 and BM36·1 cells incubated with 1 mm rNP derived from influenza virus A/HK/2/68 in the presence or absence of lactacystin, brefeldin A, chloroquine or NH₄Cl were used as stimulator cells in LST with the NP/B27 CTL (d). LST were performed in triplicate. Mean stimulation indices are shown.