Fig. 4.

Intracellular MIF and TNF-α expression in small intestinal epithelial cells from a control donor (row a) and a coeliac patient (row b). Epithelial cells were surface labelled with FITC-conjugated anti-Ber-EP4 and quadrants set on the basis of an FITC/PE conjugated control antibody. In column 1, the percentage of epithelial cells staining positive for the control antibody (PE-conjugated) is shown in the upper right quadrant. The percentage of MIF or TNF-α positive epithelial cells is shown in the upper right quadrants of the dot plots in columns 2 and 3, respectively.