Table 2.

Intracellular expression of MIF and TNF-α in colonic epithelial cell lines

	MIF		TNF-α
	% Ber-EP4+ cells	MFI	% Ber-EP4+ cells	MFI
SW 480	70	417	0	0
HCT	43	209	not done
HT-29	93	1309	36	46
LOVO	98	1963	not done
T84	79	392	0	0

Data show the intracellular expression of MIF and TNF-α protein expression in a number of different colonic epithelial cell lines. The cell lines were grown to confluence, harvested by standard trypsinization and stained for intracellular cytokine expression as described in the Patients and methods. Epithelial cells were surface labelled with anti-Ber-EP4 and the results are shown as the percentage of cytokine positive cells and median fluorescence intensity (MFI). Data is representative of three experiments performed.