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. 2001 Oct;126(1):54–63. doi: 10.1046/j.1365-2249.2001.01660.x

Fig. 3.

Fig. 3

Proportions and expression levels of LFA-1 on CD45RO + and CD45RO – lymphocytes in DS patients and control groups. (a) Two-colour flow cytometric analysis of peripheral blood lymphocytes in a typical experiment. The proportions of CD45RO + lymphocytes and CD45RO – lymphocytes were measured. Single positive population for LFA-1 (left upper quadrant) and double positive population (right upper quadrant) of lymphocytes for CD45RO/LFA-1 staining were analysed for the level of LFA-1 expression. (b) The percentage of CD45RO + expressing cells in lymphocytes were analysed in younger control children (▪, n = 9; mean age 6·5 months, CD45RO + 7·4 ± 1·0%), older control group (□, n = 8; mean age 8 years 1 month, CD45RO + 21·8 ± 1·1%), younger DS patient group (▪, n = 5; mean age 5·4 months, CD45RO + 21·1 ± 2·9%), and older DS patient group (□, n = 8, mean age 7 years 6 months, CD45RO + 36·5 ± 6·0%). (c) The expression of LFA-1 on lymphocytes on CD45RO – (left panel) and CD45RO + (right panel) expressing lymphocytes were analysed on the younger control children (LFA-1 MFI medians on CD45RO – cells and CD45RO + cells: 131 and 202), older control group (LFA-1 MFI medians on CD45RO – cells and CD45RO + cells: 143 and 332), younger DS patient group (LFA-1 MFI medians on CD45RO – cells and CD45RO + cells: 294 and 398), and older DS patient group (LFA-1 MFI medians on CD45RO – cells and CD45RO + cells: 166 and 347). Results are expressed as ranges of values, 25th percentiles, medians and 75th percentiles as in Fig. 1.