Table 1.
‘Leaky’ 8-month-old SCID mice implanted with hybridoma cells producing human monoclonal antibodies
| Mouse IgM |
Mouse IgG |
Histology |
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|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Optical density values at 410 nm |
Human IgG μg/ml |
Proteinuria (terminal values shown) |
Thrombi |
Hu IgG |
Fibrin |
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| Hybridoma | Ascites (day) | day –11 | terminal | day –11 | terminal | ascites | serum | plus score | mean arbitrary score | H&E | HRP | MSB |
| RH14 | 38 | 1·0 | 1·0 | 0·0 | 0·0 | 280 | 1254 | ++/++ + | 3·4 | + | + | + |
| 38 | 1·2 | 1·1 | 0·6 | 0·2 | 2240 | 5760 | ++/++ + | + + | + + | + + | ||
| 42 | 1·1 | 1·0 | 0·0 | 0·1 | 1103 | 23·8 | + + | – | – | – | ||
| 35 | 1·1 | 0·5 | 0·0 | 0·0 | 292 | 352 | ++/++ + | + | + | + | ||
| 34 | 0·5 | 0·6 | 0·0 | 0·0 | 464 | 1952 | ++/++ + | + | + | + | ||
| CL24 | 25 | 0·6 | 0·5 | 0·0 | 0·0 | 2916 | 1074 | + | 1·8 | – | – | – |
| 46 | 0·3 | 0·2 | 0·1 | 0·1 | 110 | 55 | trace/+ | – | – | – | ||
| 46 | 0·3 | 0·2 | 0·1 | 0·4 | 3100 | 1090 | + | – | – | – | ||
| TW | 37 | 0·7 | 0·6 | 1·4 | 1·3 | 31 | 50 | +/++ | 2·5 | – | – | – |
| 37 | 0·4 | 0·8 | 0·0 | 0·1 | 11 | 19 | + + | – | – | – | ||
| 37 | 0·7 | 0·3 | 0·0 | 0·1 | 2310 | 1660 | + | – | – | – | ||
| 37 | 0·6 | 0·3 | 0·0 | 0·1 | 2 | 4·5 | + | – | – | – | ||
| 33 | 0·4 | 0·4 | 0·0 | 0·0 | 270 | 243 | + + | – | – | – | ||
| CBF7 | 20 | 0·1 | 0·0 | 0·0 | 0·0 | 0 | 0 | + | 2 | – | – | – |
| 20 | 0·6 | 0·3 | 0·0 | 0·0 | 0 | 0 | + | – | – | – | ||
| 20 | 0·4 | 0·5 | 0·1 | 0·1 | 0 | 0 | + | – | – | – | ||
| 20 | 0·9 | 1·0 | 0·0 | 0·0 | 0 | 0 | + | – | – | – | ||
| 20 | 0·8 | 0·7 | 0·9 | 0·5 | 0 | 0 | + | – | – | – | ||
Proteinuria plus score: + = 0·3, + + = 1·0, + + + = 3·0 g/l of protein. Proteinuria arbitrary score: negative = 0, trace = 1, + = 2, + + = 3 and + + + = 4. Histology: (+) represents staining present in many glomeruli (+ +) staining present in all glomeruli in the kidney section.