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. 2001 Oct;126(1):165–172. doi: 10.1046/j.1365-2249.2001.01622.x

Fig. 1.

Fig. 1

Flow cytometric profile of hβ2m and/or HLA-G1 transfected CHO and SEC. Typical flow cytometric histograms for the HLA-G1 transfectants are shown. Naive CHO (a-c) and SEC (A-D), and stable transfectants, were treated with several specific antibodies (closed histogram), B9.12.1 (a, d, g, j, A, E, I, M), PA2.6 (b, e, h, k, B, F, J, N), BM-010 (c, f, i, l, C, G, K, O) and 74–11–10 (D, H, L, P) and isotype control antibodies (open histogram). The mean shift value of specific antibodies and control antibodies (parenthesis) are indicated in each panel. HLA-G1 expression was up-regulated by co-transfection with hβ2m in CHO-β.G1 (j, k) and SEC-β.G1 (M, N). CHO-β, CHO transfectant with hβ2m; CHO-G1, CHO transfectant with HLA-G1; CHO-β.G1, CHO transfectant with hβ2m and HLA-G1; SEC-β-1, SEC transfectant with hβ2m; SEC-G1, SEC transfectant with HLA-G1; SEC-β.G1, SEC transfectant with hβ2m and HLA-G1.