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. 2001 Oct;126(1):165–172. doi: 10.1046/j.1365-2249.2001.01622.x

Fig. 3.

Fig. 3

NK cell-mediated cytotoxicity assay. Amelioration of NK cell-mediated lysis by HLA-G1 molecule on CHO or SEC was tested as an in vitro delayed-type rejection model of a discordant xenograft. (a) Naive CHO and transfected cells were incubated with YT cells at the required E:T ratio. The results are expressed as a percentage of specific lysis. (□) Naïve CHO; (▪) CHO-G1. (b) The naive SEC and transfected cells were incubated with YT cells at the E:T ratio; 1:1. The results are expressed as a percentage of specific lysis. (□) Naïve SEC; (▪) SEC-G1; (▪) SEC-β-G1. (c) The naive SEC and two independent transfected cells with hβ2m were incubated with YT cells at the E:T ratio 6:1. The results are expressed as a percentage of specific lysis. (□) Naïve SEC; (▪) SEC-β-1; (▪) SEC-β-2.Values are presented as mean ± s.e.m. (n = 5–8). indicates a significant difference (P < 0·05). CHO-G1, CHO transfectant with HLA-G1; SEC-G1, SEC transfectant with HLA-G1; SEC-β.G1, SEC transfectant with hβ2m and HLA-G1; SEC-β, SEC transfectant with hβ2m.