NK cell-mediated cytotoxicity assay using HLA-G3 transfectants. The naive SEC or transfected cells were incubated with YT cell at the E:T ratio 6:1. The results are expressed as a percentage of specific lysis (n = 3). Naive SEC (lane 1); SEC-β-1, SEC with hβ2m (lane 2); SEC-G3, SEC with HLA-G3 (lane 3); SEC-β-1.G3, SEC with hβ2m + HLA-G3 (lane 4); SEC-β-1.FG3, SEC with hβ2m + FG3 (lane 5); SEC-β-1.FG1, SEC with hβ2m + FG1 (lane 6). Values were presented as mean ± s.e.m. Indicates significant difference (P < 0·05).