Fig. 3.

Detection of the major immediate-early gene sequence of murine cytomegalovirus by PCR in various samples. Products run on 1·0% agarose gel. Extrahepatic bile duct tissue (lane 1) and liver tissue (lane 2) removed at 14 days after intraperitoneal injection of MCMV; lane 3: pure water; lane 4: last wash of DMEM used for washing MCMV-infected biliary epithelial cells; lane 5: uninfected murine embryonic fibroblasts; lane 6: cultured biliary epithelial cells infected with MCMV in vitro; lane 7: MCMV-infected murine embryonic fibroblasts; lane 8: uninfected cultured biliary epithelial cells; M: ladder of DNA size markers; bp: base pairs.